Coding

Part:BBa_K4359006:Design

Designed by: Ira Zibbu   Group: iGEM22_IISER_TVM   (2022-10-05)


ClyA-Myc-Affi


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1003
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1003
    Illegal NheI site found at 82
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1003
    Illegal BglII site found at 159
    Illegal BglII site found at 1062
    Illegal BglII site found at 1162
    Illegal BamHI site found at 997
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1003
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1003
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This composite part was designed with an EcoN1 site upstream and BamH1 site downstream to enable ligation into the E.coli expression vector pGEX-4T1. Additional nucleotides flanking the restriction site were included to ensure efficient digestions by restriction enzymes. The sequence on the registry only includes the CDS.


Source

This part contains fragments from the E.coli genome (BBa_K4359000), engineered versions of natural proteins, (BBa_K82303) and (BBa_K4359002).

References

[1] Gujrati, V., Kim, S., Kim, S.-H., Min, J. J., Choy, H. E., Kim, S. C., & Jon, S. (2014). Bioengineered Bacterial Outer Membrane Vesicles as Cell-Specific Drug-Delivery Vehicles for Cancer Therapy. ACS Nano, 8(2), 1525–1537. https://doi.org/10.1021/nn405724x